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For transcriptome analysis, RNA was extracted from the mice ovaries (mice were 67 3.7 or 72 4.5 days old respectively at sacrifice) and purified as above, and sequenced by CEL-seq, using Illumina HiSeq 2500 at the Technion Genome Center (as in [24])

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During periods of caloric restriction, adequate protein intake reduces the degree to which the body catabolises muscle tissue for energy

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